Disruption of classical estrogenic targets in brown trout primary hepatocytes by the model androgens testosterone and dihydrotestosterone

摘要: Abstract Estrogenic effects triggered by androgens have been previously shown in a few studies. Aromatization and direct binding to estrogen receptors (ERs) are the most proposed mechanisms. For example, previously, modulation of vitellogenin A (VtgA) testosterone (T), an aromatizable androgen, was reported brown trout primary hepatocytes. The effect reversed ER antagonist. In this study, using same model disruption caused T non-aromatizable androgen - dihydrotestosterone (DHT), assessed selected estrogenic targets. Hepatocytes were exposed (96 h) six concentrations each androgen. targets VtgA, ERα, ERβ1 two zona pellucida genes, ZP2.5 ZP3a.2. aromatase CYP19a1 gene receptor (AR) also included. Modulation studied quantitative real-time PCR immunohistochemistry, HScore system. VtgA ERα up-regulated DHT (1, 10, 100 μM) (10, μM). contrast, down-regulated μM), (100 mRNA levels increased while ZP3a.2 Positive correlations found between ZPs after exposure both androgens. not changed, AR expression tended increase micromolar exposures. HScores for Vtg corroborated molecular findings. Both signaling through ERs, probably ERα.