Second primary or recurrence? Comparative patterns of p53 and K-ras mutations suggest that serous borderline ovarian tumors and subsequent serous carcinomas are unrelated tumors.

摘要: The role of serous borderline ovarian tumors (BOTs) in the pathogenesis carcinomas is unclear. Some authors have compared mutations BOTs to those carcinomas, but data on two common oncogenes, p53 and K-ras, remain inconclusive. To further clarify relationship between tumors, we performed mutational analysis from a set eight patients who first presented with advanced-stage later developed grade 1 carcinomas. Epithelium subsequent papillary was microdissected retrieved using PixCell laser-capture microscope. Stroma dissected as an internal control. DNA extracted proteinase K analyzed by single-strand conformational polymorphism-PCR for K-ras mutations. Bands altered motility were direct cycle sequencing. Seven demonstrated different secondary tumor primary tumor. For three patients, identified that absent suggesting nonclonal origin These findings are consistent hypothesis represent distinct pathological entity epithelial carcinoma.