Brain Creatine Kinase with Aging in F-344 Rats: Analysis by Saturation Transfer Magnetic Resonance Spectroscopy

作者: C.D Smith , W Landrum , J.M Carney , P.W Landfield , M.J Avison

DOI: 10.1016/S0197-4580(97)00156-5

关键词:

摘要: We measured in vivo forward flux of the creatine kinase reaction rat forebrain young (Y: 6 month, n = 13), mid-aged (M: 12 7) and aged (O: 27 10) animals using 31P magnetic resonance saturation transfer. Forward was reduced rats 0.42 +/- 0.08; M: 0.41 0.10; O: 0.31 0.03 s(-1) SD; p 0.008 O vs. Y). In vitro studies a subset same showed parallel decline CK activity 2.16 0.40; 2.17 0.25; 1.56 0.06 IU S.D.; 0.002 The spectroscopic biochemical measures were significantly correlated. Reduced could account for observed decreased aging brain. Intracellular pH, phosphocreatine/inorganic phosphate ratio, phospocreatine/gamma-adenosine triphosphate ratio did not differ between groups. may represent better measure brain energy function than relative phosphocreatine or adenosine levels observable vivo.

参考文章(37)
Britton Chance, William W. Bank, Cheng-dou Zhang, Instabilities of metabolic regulations in aging Free Radicals and Aging. ,vol. 62, pp. 58- 63 ,(1992) , 10.1007/978-3-0348-7460-1_6
Michael Simic, None, Oxygen radicals in biology and medicine Plenum Press. ,(1988) , 10.1007/978-1-4684-5568-7
C.N. Oliver, B.W. Ahn, E.J. Moerman, S. Goldstein, E.R. Stadtman, Age-related changes in oxidized proteins. Journal of Biological Chemistry. ,vol. 262, pp. 5488- 5491 ,(1987) , 10.1016/S0021-9258(18)45598-6
Bo K. Siesjö, Brain energy metabolism ,(1978)
R S Balaban, H L Kantor, J A Ferretti, In vivo flux between phosphocreatine and adenosine triphosphate determined by two-dimensional phosphorous NMR. Journal of Biological Chemistry. ,vol. 258, pp. 12787- 12789 ,(1983) , 10.1016/S0021-9258(17)44036-1
Allen C. Bowling, M.Flint Beal, Bioenergetic and oxidative stress in neurodegenerative diseases. Life Sciences. ,vol. 56, pp. 1151- 1171 ,(1995) , 10.1016/0024-3205(95)00055-B
J. A. Stolk, J. I. Oolsen, D. W. Alderman, M. P. Schweizer, Effects of age on apparent 31P spin–lattice relaxation times of rat brain phosphates Magnetic Resonance in Medicine. ,vol. 5, pp. 78- 82 ,(1987) , 10.1002/MRM.1910050110
Ronald J. T. Corbett, Abbot R. Laptook, Age-Related Changes in Swine Brain Creatine Kinase-Catalyzed 31P Exchange Measured In vivo Using 31P NMR Magnetization Transfer Journal of Cerebral Blood Flow & Metabolism. ,vol. 14, pp. 1070- 1077 ,(1994) , 10.1038/JCBFM.1994.140
Bruce N. Ames, Mark K. Shigenaga, Tory M. Hagen, Mitochondrial decay in aging Biochimica et Biophysica Acta. ,vol. 1271, pp. 165- 170 ,(1995) , 10.1016/0925-4439(95)00024-X