Quantification of Epstein-Barr Virus and Human Cytomegalovirus in Chronic Periodontal Patients

摘要: Background: Although studies focused mainly on the identification of periopathogenic bacteria, recent reports have suggested that various herpes viruses may also be involved in occurrence and progression different forms periodontal diseases. Objectives: This study aimed to compare prevalence load Epstein-Barr Virus (EBV) Human Cytomegalovirus (HCMV) subgingival tissue specimens between chronic periodontitis healthy sites. Patients Methods: A total 60 samples from systematically patients with participated this (mean age, 35 ± 7). Clinical evaluation included plaque index (PI) (Loe Silness), bleeding probing (BOP) (O’Leary), index, pocket depth (PPD) clinical attachment level measurement. Tissue harvested > 6 mm pockets ≤ 3 sulcus a quadrant same patient using curettes. Moreover, unstimulated whole saliva was gathered as shedding medium. Taq-man Real-Time Polymerase Chain Reaction assay used identify genomic copies HCMV EBV. Data were analyzed by Wilcoxon-signed ranks Friedman tests SPSS 16 software. Results: Out tissues taken periodontitis, EBV count highest least sample PD (P < 0.05). Conclusions: According results study, quantification observed is high severe periodontitis.