Oxidative Stress Induces Protein and DNA Radical Formation in Follicular Dendritic Cells of the Germinal Center and Modulates Its Cell Death Patterns in Late Sepsis

摘要: Profound depletion of follicular dendritic cells (FDCs) is a hallmark sepsis-like syndrome, but the exact causes for ensuing cell death are unknown. The death-driven contributes to immunoparalysis and responsible most morbidity mortality in sepsis. Here we have utilized immuno-spin trapping, method detection free radical formation, detect oxidative stress-induced protein DNA adducts FDCs isolated from spleen septic mice human tonsil-derived HK cells, subtype germinal center FDCs, study their role FDC depletion. At 24 h post-LPS administration, formation oxidation was significantly elevated vivo as shown by ELISA confocal microscopy. xanthine oxidase inhibitor allopurinol iron chelator desferrioxamine decreased radicals, suggesting Fenton-like chemistry formation. Protein correlated mostly with apoptotic features at necrotic morphology all types studied 48 concomitant inhibition caspase-3. cytotoxity resulted CD45R/CD138+ve plasma numbers, indicating possible defect B differentiation. In one such mechanism, initiated formed radicals which may lead FDCs.