Genistein stimulates the osteoblastic differentiation via NO/cGMP in bone marrow culture

摘要: The soybean phytoestrogen, genistein (Gen), has anabolic effects on bone through mechanisms that remain to be elucidated. We examined the role of nitric oxide (NO) and its downstream effector guanylyl cyclase (GC) in mediating Gen proliferation osteoblastic maturation primary mouse marrow-derived mesenchymal stem cells (BMSCs). (10 - 8 ∼10 6 M) resulted a dose-dependent increase cell as measured by increased [ 3 H]thymidine incorporation, stimulated assessed culture duration-dependent increments alkaline phosphatase (ALP) activity, calcium deposition into extracellular matrix Runx2/Cbfa1 gene expression BMSCs cultures. also NO synthase (NOS) formation, cGMP production were mimicked 17β-estradiol (E 2 , 10 M). Concurrent treatment with estrogen receptor (ER) antagonist ICI182,780 7 or NOS inhibitor L-NAME (3 x diminished M)-mediated production, content. In contrast, soluble GC 1 H-[1,2,4]oxadiazolo [4,3,-a]quinoxalin-1-one (ODQ, selectively blocked content but not activity. Moreover, inhibition ER, activity Gen-induced differentiation Runx2/Cbfal culture. estrogen-like stimulates at least part NO/cGMP pathway.