The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export

摘要: Abstract Background: The human immunodeficiency virus (HIV-1) uses the viral protein Rev to regulate gene expression by promoting export of unspliced and partially spliced transcripts. has been shown function in a variety organisms, including Saccharomyces cerevisiae . activity depends on nuclear signal (NES), which is believed interact either directly or indirectly with pore complex carry out its function. Crm1p member importin-beta family, other members are known be involved import. recently contribute vertebrate systems. Here, we have studied this mechanism cytoplasmic transport. Results: Viable mis-sense mutations CRM1 substantially reduced eliminated biological S. , providing strong evidence that also contributes transport NES-containing proteins ribonucleoproteins organism. interacted FG-repeat-containing as well Rev, demonstrated previously described two-hybrid interaction between yeast Rip1p dependent wild-type Crm1p. Conclusions: We conclude interacts NES during delivery cargo complex. Our findings agree current experiments orthologs Schizosaccharomyces pombe