Characterization and online detection of surfactin isomers based on HPLC-MS(n) analyses and their inhibitory effects on the overproduction of nitric oxide and the release of TNF-α and IL-6 in LPS-induced macrophages.
作者： Jin-Shan Tang , Feng Zhao , Hao Gao , Yi Dai , Zhi-Hong Yao
DOI: 10.3390/MD8102605
关键词:
摘要: A rapid method for characterization and online detection of surfactin isomers was developed based on HPLC-MS(n) (n = 1, 2, 3) analyses, many were detected characterized from the bioactive fraction mangrove bacterium Bacillus sp. Inhibitory activities overproduction nitric oxide release TNF-α IL-6 in LPS-induced macrophages systematically investigated. It revealed that showed strong inhibitory properties murine macrophage cell RAW264.7 with IC(50) values ranging 1.0 to 7.0 μM. Structure-activity relationship (SAR) studies existence free carboxyl group structure crucial. These findings will be very helpful development this novel kind natural product as new anti-inflammatory agents.
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