Bioreactor Development for Lung Tissue Engineering

摘要: Much recent interest in lung bioengineering by pulmonary investigators, industry, and the organ transplant field has seen a rapid growth of bioreactor development ranging from microfluidic scale to human-sized whole systems. Comprehension findings these models is needed provide basis for further development. The goal was comprehensively review current state lung. A search using PubMed done published, peer-reviewed papers keywords “lung” AND “bioreactor” or “bioengineering” “tissue engineering” “ex vivo perfusion”. Many new bioreactors systems have been developed both academic commercial entities. Microfluidic, lung-mimic, slice cultures advantages cost-efficiency high throughput analyses ideal pharmaceutical toxicity studies. Perfused/ventilated rodent can be adapted mid-throughput studies stem/progenitor cell development, behavior, understanding treating injury, preliminary work that translated human bioengineering. Human-sized ex incorporating perfusion ventilation are amenable automation used decellularization recellularization. Clinical preservation reconditioning currently being evaluated clinical trials. Significant advances engineering made at macroscale. most advanced closed incorporate pressure-controlled automation. Ex trials reconditioning. biggest challenges lie ahead only overcome future technology solve problems production tissue incorporation.