Enantioselective and diastereoselective separation of synthetic pyrethroid insecticides on a novel chiral stationary phase by high-performance liquid chromatography.

摘要: A novel chiral packing material for high-performance liquid chromatography (HPLC) was prepared by connecting (R)-1-phenyl-2-(4-methylphenyl) ethylamine (PTE) amide derivative of (S)-isoleucine to aminopropyl silica gel through 2-amino-3,5-dinitro-1-carboxamido-benzene unit. This stationary phase applied the enantioselective and diastereoselective separation five pyrethroid insecticides HPLC under normal condition. To achieve satisfactory baseline an optimization variables mobile composition required. The two enantiomers fenpropathrin four stereoisomers fenvalerate were separated using hexane-1,2-dichloroethane-2-propanol as phase. results show that enantioselectivity CSP is better than Pirkle type 1-A column these compounds. Only partial separations cypermethrin cyfluthrin observed. Seven peaks eight observed cyfluthrin, respectively. elution orders assigned different stereoisomer-enriched products.