Expression of transforming growth factor‐β1 in penile tissue from rats with bilateral cavernosal nerve ablation

摘要: OBJECTIVE To explore the expression of transforming growth factor-β1 (TGF-β1) in penile tissue from rats after bilateral cavernosal nerve (CN) ablation, mimicking patients who have had no nerve-sparing during prostatectomy. MATERIALS AND METHODS Ten adult male (neurectomy group) a CN resection aseptically under an operating microscope, with six sham-operated as controls. Fifteen weeks surgery apomorphine test was used all to assess erection. The specimens were then collected and prepared for detecting TGF-β1 by reverse transcription-polymerase chain reaction (RT-PCR), western blot immunohistochemistry, quantitative analysis ratio smooth muscle collagen fibres corpus cavernosum confocal microscopy. RESULTS All group but none neurectomy erectile response subcutaneous injection (100 µg/kg). Immunohistochemistry, RT-PCR analyses showed significantly higher tissues than sham surgery. Smooth cells (fluorescing red) (green autofluorescence) paraformaldehyde fixation, clearly identified microscopy. fluorescence intensity expressed mean (sem) 0.265 (0.125), lower that group, at 0.760 (0.196) (P < 0.01). CONCLUSION An increased which promotes synthesis may be one important factors dysfunction caused ablation. Similar pathophysiological processes occur radical prostatectomy.