Impact of treatment response metrics on photodynamic therapy planning and outcomes in a three-dimensional model of ovarian cancer

摘要: Common methods to characterize treatment efficacy based on morphological imaging may misrepresent outcomes and exclude effective therapies. Using a three-dimensional model of ovarian cancer, two functional response metrics are used evaluate photodynamic therapy (PDT) efficacy: total volume, calculated from viable nonviable cells, live cells. The utility these volume-based is corroborated using independent reporters activity: viability, common fluorescence-based ratiometric analysis, photosensitizer photobleaching, which characterized by loss fluorescence due in part the production reactive species during PDT. Live volume correlated with both photobleaching suggesting that it was better reporter PDT than did not correlate either metric. Based findings, viability probe susceptibilities tumor populations range dose parameters administered 0.25, 1, 10 μM benzoporphyrin derivative (BPD). 0.25 μM BPD produces most significant reduction mediates substantial shift toward small nodules. Increasingly sophisticated bioengineered models complement current planning approaches provide unique opportunities critically key including therapeutic response.