Cadmium induces interleukin-8 production in human peripheral blood mononuclear cells with the concomitant generation of superoxide radicals.

摘要: Acute or chronic exposure to cadmium (Cd) causes severe organ damages with the infiltration of leukocytes, neutrophils in particular occurring acute phase. Interleukin-8 (IL-8), a novel neutrophil chemotactic and activating cytokine, is produced by various types cells response wide variety inflammatory stimuli. The administration an antibody against IL-8 has been shown inhibit several animal models, indicating causal relationship between infiltration. Hence, this study we investigated whether Cd induced production human peripheral blood mononuclear (PBMC). Cd, over range concentrations, did induce PBMC produce large amounts bioactive IL-8, maximal induction being observed at 10(-4) M. was inhibited specifically metal chelating agent, ethylenediaminetetraacetic acid (EDTA). Steady level mRNA increased within 30 min after addition reached 2 h, decreasing thereafter. A protein synthesis inhibitor, cycloheximide, failed accumulation, that new not required for induction. Concomitantly 10 generated reactive oxygen intermediates (ROI) PBMC. radical scavenger, N-acetyl-L-cysteine (NAC), both generation ROI, implying possible involvement ROI production. This notion also supported our findings superoxide generating paraquat, NAC blocked paraquat-induced