Biocompatibility of Silver-Containing Calcium-Phosphate Cements with Anti-Bacterial Properties
作者： Yusuke Shimizu , Yusuke Kawanobe , Toshiisa Konishi , Nobuyuki Kanzawa , Michiyo Honda
DOI: 10.4028/WWW.SCIENTIFIC.NET/KEM.631.107
关键词:
摘要: We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly β-tricalcium phosphate (β-TCP) phase with anti-washout property (hereafter, β-TCP cement), which was set on basis chelate-bonding ability inositol (IP6). In this study, developed CPCs both anti-bacterial and properties adding Ag-HAp powder into above cements, examined their cytotoxicity. The Ag contents 0, 2, 5 mol% as a nominal composition were USSP raw for cement prepared ball-milling commercially-available in IP6 solution. Ag-HAp/β-TCP mixing at ratio 25:75 mass. above-mentioned 2.5 mass% Na2HPO4 solution powder/liquid 1/0.3 [g/cm3]. resulting evaluated using Staphylococcus aureus biofilm formation test. containing 2 showed strong among specimens. Furthermore, cytotoxicity Ag+ ion eluted from these also osteoblastic MC3T3-E1 cells Transwell® kit. relative cell viability cultured each Ag-containing specimen over 80 %, compared control (polystyrene plate). These results demonstrate that present are promising one candidates biocompatibility.
elsevier.com
sci-hub.se 参考文章(8)
H. Kakinuma, K. Ishii, H. Ishihama, M. Honda, Y. Toyama, M. Matsumoto, M. Aizawa, Antibacterial polyetheretherketone implants immobilized with silver ions based on chelate-bonding ability of inositol phosphate: processing, material characterization, cytotoxicity, and antibacterial properties. Journal of Biomedical Materials Research Part A. ,vol. 103, pp. 57- 64 ,(2015) , 10.1002/JBM.A.35157
D.J. Balazs, K. Triandafillu, P. Wood, Y. Chevolot, C. van Delden, H. Harms, C. Hollenstein, H.J. Mathieu, Inhibition of bacterial adhesion on PVC endotracheal tubes by RF-oxygen glow discharge, sodium hydroxide and silver nitrate treatments Biomaterials. ,vol. 25, pp. 2139- 2151 ,(2004) , 10.1016/J.BIOMATERIALS.2003.08.053
Aurora Mocanu, Gabriel Furtos, Sorin Rapuntean, Ossi Horovitz, Chirila Flore, Corina Garbo, Ancuta Danisteanu, Gheorghe Rapuntean, Cristina Prejmerean, Maria Tomoaia-Cotisel, Synthesis; characterization and antimicrobial effects of composites based on multi-substituted hydroxyapatite and silver nanoparticles Applied Surface Science. ,vol. 298, pp. 225- 235 ,(2014) , 10.1016/J.APSUSC.2014.01.166
Nusret Kose, Ali Otuzbir, Ceren Peksen, Abdurrahman Kiremitçi, Aydin Dogan, None, A Silver Ion-doped Calcium Phosphate-based Ceramic Nanopowder-coated Prosthesis Increased Infection Resistance Clinical Orthopaedics and Related Research. ,vol. 471, pp. 2532- 2539 ,(2013) , 10.1007/S11999-013-2894-X
Tom Coenye, Hans J. Nelis, In vitro and in vivo model systems to study microbial biofilm formation Journal of Microbiological Methods. ,vol. 83, pp. 89- 105 ,(2010) , 10.1016/J.MIMET.2010.08.018
Michiyo Honda, Yusuke Kawanobe, Ken Ishii, Toshiisa Konishi, Minori Mizumoto, Nobuyuki Kanzawa, Morio Matsumoto, Mamoru Aizawa, In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route Materials Science and Engineering: C. ,vol. 33, pp. 5008- 5018 ,(2013) , 10.1016/J.MSEC.2013.08.026
Toshiisa Konishi, Shuhei Takahashi, Zhi Zhuang, Kohei Nagata, Minori Mizumoto, Michiyo Honda, Yasuhiro Takeuchi, Hitomi Matsunari, Hiroshi Nagashima, Mamoru Aizawa, Biodegradable β-tricalcium phosphate cement with anti-washout property based on chelate-setting mechanism of inositol phosphate Journal of Materials Science: Materials in Medicine. ,vol. 24, pp. 1383- 1394 ,(2013) , 10.1007/S10856-013-4903-8
A. Yamamoto, R. Honma, M. Sumita, Cytotoxicity evaluation of 43 metal salts using murine fibroblasts and osteoblastic cells. Journal of Biomedical Materials Research. ,vol. 39, pp. 331- 340 ,(1998) , 10.1002/(SICI)1097-4636(199802)39:2<331::AID-JBM22>3.0.CO;2-E