Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation.

摘要: Abstract We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in mouse fibroblast cell line expressing human EGF receptors (NRHER5) and this increases dramatically upon treatment of cells physiologically relevant concentrations EGF. stimulated time-dependent increase receptor-associated PI measured immunoprecipitates. Activation was detected 15 min after addition EGF, it peaked between 1 2 hr. as low 10 pM maximal stimulation occurred at approximately nM. Analysis deacylated phosphate products, inhibition by nonionic detergent, indicated described here or 3' kinase. These results demonstrate regulation suggest potential role signal transduction pathway.