Role of nitric oxide in the epileptogenesis of EL mice.
作者： Yoshiya L. Murashima , Mitsunobu Yoshii , Jiro Suzuki
DOI: 10.1111/J.1528-1157.2000.TB01581.X
关键词:
摘要: Purpose: To understand the role of nitric oxide (NO) in regulation seizures, we measured extracellular levels NO metabolites nitrite and nitrate as indices generation parietal cortex, hippocampus, temporal cortex EL mice. Furthermore, alterations neuronal, endothelial, inducible synthetase (nNOS, eNOS, iNOS, respectively) were observed to correlate them with epileptogenesis. Methods: mice 20 weeks 30 age (before after establishment epileptogenesis, used. Nitrite was quantified using specific absorbancy diazo dye. NOS isoenzymes eNOS) also investigated hippocampus during development until old. Samples (total protein, 8.33 8.43 μg) separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified immunoblotting. Results: that experienced repetitive seizures showed a remarkable increase at compared had no experience seizures. nNOS iNOS major minor components, respectively, both increased parallel eNOS not detectable. Conclusions: Excess (and subsequent harmful NO) deficient decrease an endothelium-derived relaxing factor) may work together form focus complex.
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