Induction of VE-cadherin in rat placental trophoblasts by VEGF through a NO-dependent pathway
作者： Chih-Ching Chang , Torng-Yuo Chang , Chen-Hsiang Yu , Mei-Ling Tsai
DOI: 10.1016/J.PLACENTA.2004.06.002
关键词:
摘要: Abstract Vascular endothelial-cadherin (VE-cadherin), a calcium-dependent homotypic adhesion molecule, contributes to endothelial assembly and VEGF-mediated survival during angiogenesis. In human term placentas, villous vessels extravillous cytotrophoblasts express VE-cadherin. Therefore, the purpose of this study was examine if VEGF modulated placental development by increasing expression VE-cadherin in rat placentas. Placental tissues from rats on gestation days 14 (G14), 18 (G18) 21 (G21) were used. Western blot analysis immunohistochemistry performed detect protein abundance distribution A nitric oxide analyzer used measure released (NO) explant culture. With progression pregnancy, intensity immunoreactive staining for endovascular trophoblasts labyrinth decreased. culture, (0.01–1.0 ng/ml) increased SNP (an NO donor) or l -arginine (substrate eNOS) induced with increase production. -NAME (a NOS inhibitor) reduced VEGF-increased reversed inhibitory effect -NAME. conclusion, plays an important role induction trophoblasts, which, part, maintains trophoblast
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