Global gene expression profile of Acinetobacter baumannii during bacteremia

摘要: Background Acinetobacter baumannii is a pathogen of major importance in intensive care units worldwide, with the potential to cause problematic outbreaks and acquire high-level resistance antibiotics. There an urgent need understand mechanisms A. pathogenesis for future development novel targeted therapies. In this study we performed vivo transcriptomic analysis isolated from mammalian host bacteremia. Methods Mice were infected American Type Culture Collection 17978 using intraperitoneal injection, blood was extracted at 8 hours purify bacterial RNA RNA-Seq Illumina platform. Results Approximately one-quarter protein coding genes differentially expressed compared vitro (false discovery rate, ≤0.001; 2-fold change) 557 showing decreased 329 increased expression. Gene groups functions relating translation processing overrepresented expression, those chaperone turnover The most strongly up-regulated corresponded 3 recognized siderophore iron uptake clusters, reflecting iron-restrictive environment vivo. Metabolic changes included reduced expression involved amino acid fatty transport catabolism, indicating metabolic adaptation different nutritional environment. Genes encoding types I IV pili, quorum sensing components, proteins biofilm formation all showed Many that have been reported as essential virulence or unchanged Conclusion This provides first insight into gene profiles during life-threatening infection. Analysis regulated highlights numerous targets design therapeutics.