Acetazolamide suppresses tumor metastasis and related protein expression in mice bearing Lewis lung carcinoma.
作者： Yang Xiang , Xue-Jun Li , Bing Ma , Tao Li , He-Ming Yu
DOI:
关键词:
摘要: AIM: To study the suppressing effect of acetazolamide on tumor metastasis in vivo and observe protein alteration lung mice bearing Lewis carcinoma. METHODS: The functional role aquaporin-1 (AQP1) was investigated tumor tissues by SDS-PAGE and Western blot. tumor metastasis analyzed Lewis-lung-carcinoma model. Differential was identified SDS-PAGE, isoelectrofocusing (IEF) methods, peptide mass fingerprinting (PMF). RESULTS: Acetazolamide (40 mg/kg/d po for 21 d) dramatically reduced numbers of after sc inoculating carcinoma. inhibition rate of metastases 83.9 %. Simultaneously, AQP1 level and actin-cytoplasmic lungs containing metastatic deposits were found to be higher than that normal tissue. After treated with d, the expression obviously inhibited. CONCLUSION: can suppress metastasis, at least part, by inhibiting AQP1. actin-cytoplasmic may new prognostic molecules as well new therapeutic targets prevention and treatment tumor.
cnki.com.cn参考文章(8)
Jun Takenawa, Yoshiyuki Kaneko, Masamichi Kishishita, Hiroaki Higashitsuji, Hiroyuki Nishiyama, Toshiro Terachi, Yoichi Arai, Osamu Yoshida, Manabu Fukumoto, Jun Fujita, Transcript levels of aquaporin 1 and carbonic anhydrase IV as predictive indicators for prognosis of renal cell carcinoma patients after nephrectomy. International Journal of Cancer. ,vol. 79, pp. 1- 7 ,(1998) , 10.1002/(SICI)1097-0215(19980220)79:1<1::AID-IJC1>3.0.CO;2-5
Aigang Lu, Heming Yu, Kai Chen, S.S. Koide, Xuejun Li, Alteration in brain proteins following occlusion of the middle cerebral artery in rat. Life Sciences. ,vol. 65, pp. 493- 500 ,(1999) , 10.1016/S0024-3205(99)00270-2
J Vandekerckhove, G Bauw, K Vancompernolle, B Honoré, J Celis, Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells. Journal of Cell Biology. ,vol. 111, pp. 95- 102 ,(1990) , 10.1083/JCB.111.1.95
Hisakazu Fujita, Hiroaki Suzuki, Noboru Kuzumaki, Leonhard Müllauer, Yoshifumi Ogiso, Atsushi Oda, Ken Ebisawa, Takashi Sakurai, Yoshiaki Nonomura, Shigeko Kijimoto-Ochiai, A specific protein, p92, detected in flat revertants derived from NIH/3T3 transformed by human activated c-Ha-ras oncogene. Experimental Cell Research. ,vol. 186, pp. 115- 121 ,(1990) , 10.1016/0014-4827(90)90217-X
G. M. Preston, T. P. Carroll, W. B. Guggino, P. Agre, Appearance of Water Channels in Xenopus Oocytes Expressing Red Cell CHIP28 Protein Science. ,vol. 256, pp. 385- 387 ,(1992) , 10.1126/SCIENCE.256.5055.385
C. Casey Cunningham, Actin structural proteins in cell motility. Cancer and Metastasis Reviews. ,vol. 11, pp. 69- 77 ,(1992) , 10.1007/BF00047604
S. Parkkila, H. Rajaniemi, A.-K. Parkkila, J. Kivela, A. Waheed, S. Pastorekova, J. Pastorek, W. S. Sly, Carbonic anhydrase inhibitor suppresses invasion of renal cancer cells in vitro. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 2220- 2224 ,(2000) , 10.1073/PNAS.040554897
Jing-Lan Wang, Jing-Hong Wan, Ling Luo, Shao-Xiang Xiong, Guang-Hui Wang, Fu-Chu He, Xiao-Hong Qian, None, Development and Preliminary Application of a Peptide Mass Fingerprinting Technique in Proteome Research. Acta Biochimica et Biophysica Sinica. ,vol. 32, pp. 373- 378 ,(2000)