Assessment of X Chromosome Dosage Compensation in Caenorhabditis elegans by Phenotypic Analysis of lin-14
作者： Leslie DeLong , Lawrence P Casson , Barbara J Meyer
DOI: 10.1093/GENETICS/117.4.657
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摘要: Caenorhabditis elegans compensates for the difference in X chromosome gene dose between males (XO) and hermaphrodites (XX) through a mechanism that equalizes levels of X-specific mRNA transcripts two sexes. We have devised sensitive quantitative genetic assay to measure perturbations expression caused by mutations affect this process dosage compensation. The is based on quantitating precocious alae phenotype mutation reduces but does not eliminate function X-linked lin-14. demonstrate diploid animals lin-14 compensated XO XX animals. In XXX animals, however, compensated, implying normal compensation C. lacks capacity compensate completely additional triplo-X Using we compare effects genes dpy-21, dpy-26, dpy-27, dpy-28, dpy-22 expression. Additionally, case dpy-21 correlate change phenotypic with corresponding transcript level.
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