Nature of Particles Involved in Lipid Synthesis in Yeast

摘要: Klein, Harold P. (Ames Research Center, Moffett Field, Calif.). Nature of particles involved in lipid synthesis yeast. J. Bacteriol. 90: 227–234. 1965.—Mitochondria-free homogenates Saccharomyces cerevisiae yielded several particulate layers upon centrifugation at 100,000 × g . Electron microscopy revealed that membranes are present only the uppermost (“fluffy”) layer, which is inactive synthesis. The membrane-free material middle (“red”) layer stimulated fatty acids and nonsaponifiable lipids. In addition, this fraction appeared to be rich enzyme systems responsible for desaturating converting squalene sterols. purified contained protein ribonucleic acid (approximately 65:35), further resembled ribosomal they sedimented almost entirely as an 80 S particle tris(hydroxymethyl)aminomethane-magnesium buffer. Various treatments dissociated did not affect lipogenic capabilities fraction. Images