Role of p38MAPK in apoptosis and autophagy responses to photodynamic therapy with Chlorin e6.
作者： Qin Xue , Xiaobing Wang , Pan Wang , Kun Zhang , Quanhong Liu
DOI: 10.1016/J.PDPDT.2014.12.001
关键词:
摘要: Summary Background Photodynamic therapy (PDT) has been undergoing clinical evaluation for the treatment of colorectal cancer. But molecular mechanism photodynamic injury in human cancer cells still remains unclear. Methods Chlorin e6 (Ce6) was used to photosensitize SW620 cells. The inhibitory effect PDT evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide tetrazolium) assay and colony forming assay. Apoptosis determined nuclear DAPI (4′-6-diamidino-2-phenylindole) staining Annexin V-PE/7-AAD Monodansylcadaverine (MDC) evaluate abundance autophagic vacuoles treated apoptosis autophagy associated proteins were analyzed western blotting. Moreover, we applied siRNA p38MAPK inhibitor SB203580 dissect its on cellular response Results Ce6 mediated (Ce6-PDT) induced apparent with dependent ROS (reactive oxygen species) generation. When inhibited or SB203580, a marked enhancement detected after PDT. 3-methyladenine/Bafilomycin A1 greatly aggravated photodamage Conclusion Ce6-PDT production activate probably prevent from photodamage. Inhibition activation accelerated cell apoptosis, meanwhile enhanced may act as cytoprotective process
sci-hub.se 参考文章(39)
Paulo M. Hoff, Scott Kopetz, Daniela Freitas, Aknar F.C. Calabrich, Adjuvant Chemotherapy for Stage II Colon Cancer Oncology. ,vol. 22, pp. 260- 270 ,(2008)
Feifan Zhou, Ying Yang, Da Xing, Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis FEBS Journal. ,vol. 278, pp. 403- 413 ,(2011) , 10.1111/J.1742-4658.2010.07965.X
Timothy S. Lewis, Paul S. Shapiro, Natalie G. Ahn, Signal Transduction through MAP Kinase Cascades Advances in Cancer Research. ,vol. 74, pp. 49- 139 ,(1998) , 10.1016/S0065-230X(08)60765-4
Dennis E.J.G.J. Dolmans, Dai Fukumura, Rakesh K. Jain, Photodynamic therapy for cancer Nature Reviews Cancer. ,vol. 3, pp. 380- 387 ,(2003) , 10.1038/NRC1071
Jean-Francois Groulx, Taoufik Khalfaoui, Yannick D. Benoit, Gérald Bernatchez, Julie C. Carrier, Nuria Basora, Jean-François Beaulieu, Autophagy is active in normal colon mucosa. Autophagy. ,vol. 8, pp. 893- 902 ,(2012) , 10.4161/AUTO.19738
Olga Burmistrova, José Quintana, Jesús G Díaz, Francisco Estévez, None, Astragalin heptaacetate-induced cell death in human leukemia cells is dependent on caspases and activates the MAPK pathway. Cancer Letters. ,vol. 309, pp. 71- 77 ,(2011) , 10.1016/J.CANLET.2011.05.018
Zerihun Assefa, Annelies Vantieghem, Wim Declercq, Peter Vandenabeele, Jackie R. Vandenheede, Wilfried Merlevede, Peter de Witte, Patrizia Agostinis, The Activation of the c-Jun N-terminal Kinase and p38 Mitogen-activated Protein Kinase Signaling Pathways Protects HeLa Cells from Apoptosis Following Photodynamic Therapy with Hypericin Journal of Biological Chemistry. ,vol. 274, pp. 8788- 8796 ,(1999) , 10.1074/JBC.274.13.8788
Bao-Xiu Li, Chun-Yan Li, Rui-Qing Peng, Xiao-Jun Wu, Hai-Ying Wang, De-Sen Wan, Xiao-Feng Zhu, Xiao-Shi Zhang, The expression of beclin 1 is associated with favorable prognosis in stage IIIB colon cancers Autophagy. ,vol. 5, pp. 303- 306 ,(2009) , 10.4161/AUTO.5.3.7491
James M. Olson, Andrew R. Hallahan, p38 MAP kinase: a convergence point in cancer therapy Trends in Molecular Medicine. ,vol. 10, pp. 125- 129 ,(2004) , 10.1016/J.MOLMED.2004.01.007
Zerihun Assefa, Annelies Vantieghem, Marjan Garmyn, Wim Declercq, Peter Vandenabeele, Jackie R. Vandenheede, Roger Bouillon, Wilfried Merlevede, Patrizia Agostinis, p38 mitogen-activated protein kinase regulates a novel, caspase-independent pathway for the mitochondrial cytochrome c release in ultraviolet B radiation-induced apoptosis. Journal of Biological Chemistry. ,vol. 275, pp. 21416- 21421 ,(2000) , 10.1074/JBC.M002634200