lncRNA TINCR participates in ALA-PDT-induced apoptosis and autophagy in cutaneous squamous cell carcinoma.

摘要: This study aims to investigate whether terminal differentiation-induced ncRNA (TINCR) has an effect on apoptosis and autophagy induced by ALA-PDT in cutaneous squamous cell carcinoma (CSCC). A431 cells were treated with 5-aminolevulinic acid (ALA) solution at different concentrations for duration time. viability was detected Cell Counting Kit-8 (CCK-8) assay, relative TINCR messenger RNA expression quantitative reverse-transcription polymerase chain reaction (qRT-PCR). examined flow cytometry. Relative apoptosis/autophagy-related protein analyzed Western blot analysis. The of RFP-LC3 immunofluorescence assay. Reactive oxygen species concentration 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe. expressions ERK1/2 specificity 3 (Sp3) A43 analysis qRT-PCR. Sp3 binding sites ChIP-qPCR. transcription activity measured luciferase reporter treatment 3.2 mmol/L 120 minutes significantly promoted CSCC cells, ALA-PDT-induced autophagy. Furthermore, through ERK1/2-SP3 pathway. promoters. Our data indicated that involves CSCC.