A Simple Electrochemical Aptamer Cytosensor for Direct Detection of Chronic Myelogenous Leukemia K562 Cells
作者： Tianxiao Yu , Hui Zhang , Zhenglan Huang , Zhenhong Luo , Ningshu Huang
关键词:
摘要: A simple and rapid electrochemical aptamer cytosensor has been developed for direct detection of chronic myelogenous leukemia (CML) K562 cells based on a specific biotin conjugated concanavalin (bio-ConA) probe. The cell could be specifically recognized by T2-KK1B10 capture pre-immobilized gold modified electrode surface. Then, bio-ConA was added in the reaction to identify surface mannose, resulting an aptamer-K562 cell-bio-ConA sandwich complex. Finally, streptavidin alkaline phosphatase (ST-ALP) combined with catalyze α-naphthyl (α-NP) phosphate form α-naphthol which is highly electroactive at operating voltage 180 mV (vs. Ag/AgCl). Under optimum conditions, DPV signals were proportional logarithm from 1×102 1×107 cells mL−1 limit 79 cells mL−1. also exhibited high selectivity, stability reproducibility. When applied detect human blood samples, recoveries between 79.6 %–93.3 % obtained, indicating biosensor would potential alternative tool CML real biological samples.
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