Immunoassay of the adenosine deaminase complexing proteins of human tissues and body fluids.

摘要: A sensitive immunoassay for the adenosine deaminase binding protein (complexing protein) of human kidney has been developed. Impetus development assay was provided by observations that (a) antibody to complexing does not react with catalytically active monomer, and (b) affect or catalytic activity enzyme monomer. Preformed immune precipitate prepared from rabbit anti-kidney serum goat anti-rabbit gamma-globulin is used selectively insolubilize protein. Quantitation accomplished measuring intrinsic deaminating capacity held in precipitate. As little as 1 ng can be accurately quantitated assay. The demonstrate proteins liver, lung, spleen, fibroblasts, plasma, urine shared bind coupled their antigenic similarity suggests a number tissues body fluids may products same gene.