Pharmacodynamics of Streptavidin-Coated Cyanoacrylate Microbubbles Designed for Molecular Ultrasound Imaging

作者: Moritz Palmowski , Bernd Morgenstern , Peter Hauff , Michael Reinhardt , Jochen Huppert

DOI: 10.1097/RLI.0B013E31815A251B

关键词:

摘要: Objectives: To assess the pharmacodynamic behavior of cyanoacrylate, streptavidin-coated microbubbles (MBs) and to investigate their suitability for molecular ultrasound imaging. Materials Methods: Biodistribution MBs was analyzed in tumor-bearing mice using γ-counting, immunohistochemistry, flow cytometry, ultrasound. Further, vascular endothelial growth factor receptor 2-antibody coupled were used image tumor neovasculature. Results: After 1 minute >90% cleared from blood pooled lungs, liver, spleen. Subsequently, within hour a decent reincrease MB-concentration observed blood. The remaining removed by liver spleen macrophages. About 30% phagocytosed intact after 48 hours. Shell fragments found kidneys only. No relevant MB-accumulation tumors. In contrast, 2-specific accumulated significantly vasculature (P Conclusions: pharmacokinetic cyanoacrylate has been studied. this context, low amount tumors >5 minutes is beneficial specific targeting angiogenesis.

参考文章(25)
Reba Rc, McAfee Jg, Atkins Hl, Gagne G, Smith Em, Kirchner Pt, Blaufox, Biological distribution and excretion of DTPA labeled with Tc-99m and In-111. The Journal of Nuclear Medicine. ,vol. 20, pp. 1273- 1278 ,(1979)
NM GREEN, AVIDIN. 3. THE NATURE OF THE BIOTIN-BINDING SITE. Biochemical Journal. ,vol. 89, pp. 599- 609 ,(1963) , 10.1042/BJ0890599
Douglas J. Wagenaar, Ralph Weissleder, Arne Hengerer, Glossary of molecular imaging terminology. Academic Radiology. ,vol. 8, pp. 409- 420 ,(2001) , 10.1016/S1076-6332(03)80549-4
Jonathan R. Lindner, Paul A. Dayton, Matthew P. Coggins, Klaus Ley, Ji Song, Katherine Ferrara, Sanjiv Kaul, Noninvasive Imaging of Inflammation by Ultrasound Detection of Phagocytosed Microbubbles Circulation. ,vol. 102, pp. 531- 538 ,(2000) , 10.1161/01.CIR.102.5.531
Jonathan R. Lindner, Microbubbles in medical imaging: current applications and future directions. Nature Reviews Drug Discovery. ,vol. 3, pp. 527- 532 ,(2004) , 10.1038/NRD1417
Miles Lankford, Carolyn Z. Behm, James Yeh, Alexander L. Klibanov, Peter Robinson, Jonathan R. Lindner, Effect of microbubble ligation to cells on ultrasound signal enhancement: implications for targeted imaging. Investigative Radiology. ,vol. 41, pp. 721- 728 ,(2006) , 10.1097/01.RLI.0000236825.72344.A9
Ralf A. Linker, Michael Reinhardt, Martin Bendszus, Gesa Ladewig, Andreas Briel, Michael Schirner, Mathias Mäurer, Peter Hauff, In vivo molecular imaging of adhesion molecules in experimental autoimmune encephalomyelitis (EAE) Journal of Autoimmunity. ,vol. 25, pp. 199- 205 ,(2005) , 10.1016/J.JAUT.2005.09.019
Gregory M. Lanza, Kirk D. Wallace, Michael J. Scott, William P. Cacheris, Dana R. Abendschein, Donald H. Christy, Angela M. Sharkey, James G. Miller, Patrick J. Gaffney, Samuel A. Wickline, A Novel Site-Targeted Ultrasonic Contrast Agent With Broad Biomedical Application Circulation. ,vol. 94, pp. 3334- 3340 ,(1996) , 10.1161/01.CIR.94.12.3334