An immunoassay for the detection and quantitative determination of mycothiol

摘要: Mycothiol (MSH) is a glycosylated derivative of N-acetylcysteine that may have antioxidant functions in mycobacteria and other actinomycetes. To develop highly specific assay for MSH, we capitalized on the selective binding thiols to maleimide residue linked bovine serum albumin employed affinity-purified polyclonal antibody an enzyme-linked secondary detection. The was shown be detect MSH at levels as low 0.1 pmol when conducted form microtiter plate-based ELISA. A similar, nitrocellulose membrane-based immunoassay useful qualitative detection MSH-producing bacterial colonies.