The feedback regulator Nord controls Dpp/BMP signaling via extracellular interaction with Dally in theDrosophilawing

摘要: Abstract The Drosophila BMP 2/4 homologue Decapentaplegic (Dpp) acts as a morphogen to regulate diverse developmental processes, including wing morphogenesis. Transcriptional feedback regulation of this pathway ensures tightly controlled signaling outputs generate the precise pattern adult wing. Nevertheless, few direct Dpp target genes have been explored and our understanding remains incomplete. Here, we employ transcriptional profiling following dpp conditional knockout identify nord, novel Dpp/BMP regulator. Nord mutants generated by CRISPR/Cas9 mutagenesis produce smaller display low penetrance venation defects. At molecular level, nord encodes heparin-binding protein show that its overexpression is sufficient antagonize signaling. Further, demonstrate physically genetically interacts with co-receptor Dally. In sum propose Dally fine tune signaling, implications for both disease models. Impact statement Functional analyses Neuron Derived Neurotrophic Factor reveal new mode extracellular heparan sulfate proteoglycan required proper action.