NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES

摘要: A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion rat homogenate. The factor is no longer antigenic or at most only slightly so. It stable 60 degrees , destroyed boiling, non-dialyzable, precipitated ammonium sulfate, but resists destruction proteolytic and other enzymes. In accord with previous studies on the organ localization tissue antigen responsible for production antibodies, derivative, lung, skeletal muscle, heart, liver.