Cloning and Analysis of the l-Lactate Utilization Genes from Streptococcus iniae
作者： A Gibello , MD Collins , L Dominguez , JF Fernandez-Garayzabal , PT Richardson
DOI: 10.1128/AEM.65.10.4346-4350.1999
关键词:
摘要: The presence of lactate oxidase was examined in eight Streptococcus species and some related bacteria. A clone (pGR002) isolated from a genomic library iniae generated Escherichia coli, containing DNA fragment spanning two genes designated lctO lctP. We show that these are likely to be involved the l-lactic acid aerobic metabolism this organism. This has been sequenced characterized. comparison deduced amino sequence LctP protein demonstrated had significant homology with l-lactate permeases other LctO S. also showed strong Aerococcus viridans NAD-independent dehydrogenases, all belonging family flavin mononucleotide-dependent α-hydroxyacid-oxidizing enzymes. Biochemical assays gene products confirm identity reveal possible role for iniae. is discussed relation growth organism response carbon source availability.
unirioja.es
sci-hub.st 参考文章(33)
Alan J. Hillier, G. Richard Jago, L-Lactate dehydrogenase, FDP-activated, from Streptococcus cremoris. Methods in Enzymology. ,vol. 89, pp. 362- 367 ,(1982) , 10.1016/S0076-6879(82)89065-4
S F Kim, S J Baek, M Y Pack, Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene. Applied and Environmental Microbiology. ,vol. 57, pp. 2413- 2417 ,(1991) , 10.1128/AEM.57.8.2413-2417.1991
M Volokita, C R Somerville, The primary structure of spinach glycolate oxidase deduced from the DNA sequence of a cDNA clone. Journal of Biological Chemistry. ,vol. 262, pp. 15825- 15828 ,(1987) , 10.1016/S0021-9258(18)47662-4
D A Giegel, C H Williams, V Massey, L-lactate 2-monooxygenase from Mycobacterium smegmatis. Cloning, nucleotide sequence, and primary structure homology within an enzyme family. Journal of Biological Chemistry. ,vol. 265, pp. 6626- 6632 ,(1990) , 10.1016/S0021-9258(19)39195-1
J M Dong, J S Taylor, D J Latour, S Iuchi, E C Lin, Three overlapping lct genes involved in L-lactate utilization by Escherichia coli. Journal of Bacteriology. ,vol. 175, pp. 6671- 6678 ,(1993) , 10.1128/JB.175.20.6671-6678.1993
Makoto Ono, Hiroshi Matsuzawa, Takahisa Ohta, Nucleotide sequence and characteristics of the gene for L-lactate dehydrogenase of Thermus aquaticus YT-1 and the deduced amino acid sequence of the enzyme. Journal of Biochemistry. ,vol. 107, pp. 21- 26 ,(1990) , 10.1093/OXFORDJOURNALS.JBCHEM.A123004
E I Garvie, Bacterial lactate dehydrogenases. Microbiological Research. ,vol. 44, pp. 106- 139 ,(1980) , 10.1128/MR.44.1.106-139.1980
Florence LEDERER, Sylvie CORTIAL, Anne-Marie BECAM, Pierre-Yves HAUMONT, Lourdes PEREZ, Complete amino acid sequence of flavocytochrome b2 from baker's yeast FEBS Journal. ,vol. 152, pp. 419- 428 ,(1985) , 10.1111/J.1432-1033.1985.TB09213.X
Robert D Fleischmann, Mark D Adams, Owen White, Rebecca A Clayton, Ewen F Kirkness, Anthony R Kerlavage, Carol J Bult, Jean-Francois Tomb, Brian A Dougherty, Joseph M Merrick, Keith McKenney, Granger Sutton, Will FitzHugh, Chris Fields, Jeannine D Gocayne, John Scott, Robert Shirley, Li-lng Liu, Anna Glodek, Jenny M Kelley, Janice F Weidman, Cheryl A Phillips, Tracy Spriggs, Eva Hedblom, Matthew D Cotton, Teresa R Utterback, Michael C Hanna, David T Nguyen, Deborah M Saudek, Rhonda C Brandon, Leah D Fine, Janice L Fritchman, Joyce L Fuhrmann, NSM Geoghagen, Cheryl L Gnehm, Lisa A McDonald, Keith V Small, Claire M Fraser, Hamilton O Smith, J Craig Venter, Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. ,vol. 269, pp. 496- 512 ,(1995) , 10.1126/SCIENCE.7542800
Reinhard HENSEL, Ulrich MAYR, Hirota FUJIKI, Otto KANDLER, Comparative Studies of Lactate Dehydrogenases in Lactic Acid Bacteria Amino‐Acid Composition of an Active‐Site Region and Chemical Properties of the L‐Lactate Dehydrogenase of Lactobacillus casei, Lactobacillus curvatus, Lactobacillus plantarum, and Lactobacillus acidophilus FEBS Journal. ,vol. 80, pp. 83- 92 ,(1977) , 10.1111/J.1432-1033.1977.TB11859.X