MiR-221-3p is down-regulated in preeclampsia and affects trophoblast growth, invasion and migration partly via targeting thrombospondin 2

摘要: Preeclampsia (PE) is a common obstetrical disorder characterized by hypertension and proteinuria. The aberrant expression of miR-221-3p in placental tissues has been implicated the pathogenesis PE. This study examined role trophoblast growth, invasion migration, explored underlying mechanisms. Our results showed that was down-regulated from PE patients compared to heathy controls as measured quantitative real-time PCR assay. CCK-8 assay, Transwell assay wound healing overexpression promoted (HTR-8/SVneo cells) knockdown exerted opposite effects. Flow cytometry experiments further demonstrated inhibited apoptosis, increased cell population at S phase, decreased G0/G1 phase HTR-8/SVneo cells; while Bioinformatics prediction luciferase reporter targeted 3' untranslated region thrombospondin 2 (THBS2), qRT-PCR western blot assays revealed negatively regulated THBS2 cells. Furthermore, attenuated vitro effects on migration clinical sample analysis mRNA levels significantly correlated with miR-221-3p. In summary, our PE, functional partly via targeting THBS2.