Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets
作者： Todd J. Brinkman , David K. Person , Michael K. Schwartz , Kristine L. Pilgrim , Kevin E. Colson
DOI: 10.1007/S12686-010-9176-7
关键词:
摘要: We tested a protocol for extracting DNA from fecal pellets Sitka black-tailed deer (Odocoileus hemionus sitkensis) and evaluated genotyping performance of previously developed microsatellite markers as well suite new designed specifically this study. screened 30 microsatellites, identified 7 (23%) loci including 4 markers, that fit into single multiplex consistently genotyped with low error rates. was extracted 2,408 fecal-pellet samples. Of those, 1,240 (52%) were successfully at all allowing identification 634 genetically unique deer. Using collected in the field an effective technique identifying distinguishing among
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