Sodium dodecyl sulfate-gel electrophoresis of proteins employing short capillaries.

摘要: The performance of capillary gel-sodium dodecyl sulfate (SDS) electrophoresis for molecular-mass-dependent separation proteins has been examined employing cross-linked polyacrylamide gels and untreated fused-silica capillaries short length. An apparatus constructed, combining a UV detector equipped with holder, power supply, micromanipulator sample loading. is capable accommodating gel different lengths can be operated two methods loading: manual injection on top the or electrokinetic injection. Standard have separated according to their molecular mass (ranging from 17000 116000) within 30 min, effective shorter than 10 cm ranging 2.4 4.8%T (5%C). results confirmed that length less used protein size-separation by SDS electrophoresis, much higher achieved in rod gel-SDS electrophoresis.