Size separation of sodium dodecyl sulfate complexes of human plasma proteins by capillary electrophoresis employing linear polyacrylamide as a sieving polymer
作者： Takashi Manabe , Hideto Oota , Jun Mukai
关键词: Polyacrylamide gel electrophoresis 、 Capillary electrophoresis 、 Blood proteins 、 Molecular mass 、 Sodium dodecyl sulfate 、 Gel electrophoresis of proteins 、 Chemistry 、 Chromatography 、 Gel electrophoresis 、 Polyacrylamide
摘要: Electrophoretic conditions to separate sodium dodecyl sulfate (SDS) complexes of human plasma proteins according their size differences, by capillary electrophoresis employing linear polyacrylamide as a sieving matrix (LPA-CE), have been examined. Using the optimized separation conditions, SDS not treated with reducing agents were separated into about 40 peaks and shoulders within 60 min. The molecular mass values major in pattern estimated from plot migration time for standard some identified on pattern. electrophoretic successfully applied analysis immunoglo-bulin G (IgG) myeloma sera.
sci-hub.se 参考文章(21)
L Sottrup-Jensen, T M Stepanik, T Kristensen, D M Wierzbicki, C M Jones, P B Lønblad, S Magnusson, T E Petersen, Primary structure of human alpha 2-macroglobulin. V. The complete structure. Journal of Biological Chemistry. ,vol. 259, pp. 8318- 8327 ,(1984) , 10.1016/S0021-9258(17)39730-2
Rosalind Pitt-Rivers, F. S. Ambesi Impiombato, The binding of sodium dodecyl sulphate to various proteins Biochemical Journal. ,vol. 109, pp. 825- 830 ,(1968) , 10.1042/BJ1090825
T Manabe, E Hayama, T Okuyama, Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine. Clinical Chemistry. ,vol. 28, pp. 824- 827 ,(1982) , 10.1093/CLINCHEM/28.4.824
Stellan Hjertén, Ming-de Zhu, Adaptation of the equipment for high-performance electrophoresis to isoelectric focusing Journal of Chromatography A. ,vol. 346, pp. 265- 270 ,(1985) , 10.1016/S0021-9673(00)90512-0
S. K. Karathanasis, V. I. Zannis, J. L. Breslow, Isolation and characterization of the human apolipoprotein A-I gene. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 6147- 6151 ,(1983) , 10.1073/PNAS.80.20.6147
Takashi Manabe, Sophia Visvikis, Josiane Steinmetz, Marie M. Galteau, Tsuneo Okuyama, Gérard Siest, Systematic analysis of serum lipoproteins and apolipoproteins by a combined technique of micro two-dimensional electrophoresis Electrophoresis. ,vol. 8, pp. 325- 330 ,(1987) , 10.1002/ELPS.1150080706
Takashi Manabe, Aiko Iwasaki, Hiromitsu Miyamoto, Separation of human plasma/serum proteins by capillary isoelectric focusing in the absence of denaturing agents Electrophoresis. ,vol. 18, pp. 1159- 1165 ,(1997) , 10.1002/ELPS.1150180723
András Guttman, Judith A. Nolan, Nelson Cooke, Capillary sodium dodecyl sulfate gel electrophoresis of proteins Journal of Chromatography A. ,vol. 632, pp. 171- 175 ,(1993) , 10.1016/0021-9673(93)80041-6
Takashi Manabe, Sodium dodecyl sulfate-gel electrophoresis of proteins employing short capillaries. Electrophoresis. ,vol. 16, pp. 1468- 1473 ,(1995) , 10.1002/ELPS.11501601243
Takashi Manabe, Hiromitsu Miyamoto, Aiko Iwasaki, Effects of catholytes on the mobilization of proteins after capillary isoelectric focusing Electrophoresis. ,vol. 18, pp. 92- 97 ,(1997) , 10.1002/ELPS.1150180118