Immunohistochemical Analysis of Spontaneous Pancreatic Islet Amyloid Deposits in Nonhuman Primates

摘要: Pancreatic islet amyloidosis has been previously reported in aged diabetic and nondiabetic humans, nonhuman primates, cats, raccoons (Procyon lotor), degus (Ocrodon degus, a South American rodent)."h In amyloidosis, often accompanied by diabetes mellitus, Celebes crested macaques (Macaca nigYU)~ rhesus monkeys ~nulatta), cynomolgus fascicularis), Formosan rock macaque cyclopis), drill baboon (Mandrillus leucophams). 1.2.7.8 Human feline amyloid deposits are derived from the polymerization of polypeptide (IAPP; amylin), whereas degu, they insulin."69 IAPP is an beta-cell product secreted with insulin humans many other ~pecies.~ The definitive biological functions significance pathogenesis human type 2 (non-insulin dependent) mellitus unre~olved.~.~-~ purpose this study was to examine immunoreactivity pancreatic captive primates. A review necropsy records several sources identified four monkeys, three macaques, one orangutan (Pongo pygmaeus) deposits. All primates were adults ranging 7 26 years age. One had but metabolic status animals not known. Formalin-fixed, paraffin-embedded tissues sectioned at 5 lm stained either hematoxylin eosin (HE) or Congo red without KMnO, pretreatment. red-stained sections examined both bright field polarized light microscopy. Using modified avidin-biotin-peroxidase complex (ABC) method,> evaluated for guinea pig anti-porcine serum (Dako Corp., Carpinteria, CA) diluted 1 : 800 phosphate-buffered saline (PBS), pH 7.4; 400 rabbit anti-human glucagon (Dako); 100 somatostatin calcitonin gene-related peptide (CGRP) (Peninsula Laboratories, Belmont, CA); 200 amylin (Peninsula). Positive controls included internal tissue containing application primary antisera normal bonnet radiata) pancreas. Negative replacing appropriate nonimmune sera applying canine kidney glomerular amyloid. Five-micrometer collected on positively charged glass slides (Fisher Scientific, Pittsburgh, PA), deparaffinized, rehydrated, incubated 1.5% goat PBS 20 minutes room temperature humidified chamber. Sections antibody hour under same conditions, followed 30-minute incubation 3% hydrogen peroxide methanol block endogenous peroxidase activity incubations biotinylated anti-rabbit anti-guinea IgG (Vector Burlingame, CA), ABC (Vector). chromagen 0.0 16% diaminobenzidine tetrahydrochloride (Sigma Chemical Co., St. Louis, MO) NiCI, (Digene Diagnostics, Silver Spring, MD) 0.024% PBS. counterstained nuclear fast (Digene). multifocal diffuse amyloidosis. extent distribution ranged mild deposition surrounding capillaries little no loss cells marked enlarged islets severely reduced