Localisation of hydrogen peroxide and peroxidase in gametophytes of Ceratopteris richardii (C-fern) grown in the presence of pathogenic fungi in a gnotobiotic system

摘要: The endogenous localisation of peroxidase and hydrogen peroxide (H 2 O ) was detected when gametophytes the fern, Ceratopteris richardii, were exposed to plant pathogenic fungi Sclerotium rolfsii Sclerotinia sclerotiorum Phytophthora infestans, an oomycete, in a gnotobiotic system. This accomplished by light microscopy using 3,3'-diaminobenzidine, guaiacol H starch potassium iodide (KI) staining procedures, which facilitated observation reaction vivo situ, without physically damaging tissues. All three methods promoted at rhizoid regions. Although most cells destroyed S. sclerotiorum, there where mycelial growth confluent with cell walls. A qualitative test confirmed that colour change KI agar medium, as well histochemical KI, because secreted or not oxalic acid. When P. no infection occurred, but irrespective tested. Based on grown presence it is possible plants coupled may prevent invasion nonpathogens functioning barrier. fern-pathogen model system has potential for application tool study host-parasite interaction