PEGylated J591 mAb loaded in PLGA-PEG-PLGA tri-block copolymer for targeted delivery: in vitro evaluation in human prostate cancer cells.
作者： Stanley Moffatt , Richard J Cristiano , None
DOI: 10.1016/J.IJPHARM.2006.04.011
关键词:
摘要: J591 monoclonal antibody (mAb) has high affinity for prostate specific membrane antigen (PSMA) on cancer (PCA) cells. We coupled polyethylene glycol-J591 (PEGylated J591) to a salicyl hydroxamic acid (SHA)-derivatized polyethylenimine (PEI)/DNA-betagal vector investigate the specificity and efficiency of targeting PSMA in PCA cells through encapsulation. Coupling was facilitated via interaction between phenyl(di)boronic (PDBA) SHA molecules yielding J591/PEG/PEI/DNA-betagal polyplex. After encapsulation with poly(d,l-lactic-co-glycolic acid)-b-polyethylene glycol-b-poly(d,l-lactic-co-glycolic acid) (PLGA-PEG-PLGA) tri-block copolymer, 8-10-fold increment gene transfection levels were attained at optimum concentration 0.25% (w/v) using Pluronic F68 copolymer as control. The enhanced attributed increased internalization uptake radiolabeled plasmid presence PLGA-PEG-PLGA copolymer. release DNA (pDNA) from microparticles containing SHA-PEI-complexed pDNA showed little initial burst followed by 5% over 48 h. accelerated thereafter approximately 60% released after 28 days. Deconvolution confocal microscopy polyplex/microparticle formulation localized cell nucleus opposed polyplex without indicating that an optimal can be utilized enhance endocytic process J591-mediated
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