In vitro degradation and release profiles of poly-DL-lactide-poly(ethylene glycol) microspheres with entrapped proteins

作者: Xiaohong Li , Xianmo Deng , Minglong Yuan , Chengdong Xiong , Zhitang Huang

DOI: 10.1002/1097-4628(20001003)78:1<140::AID-APP180>3.0.CO;2-P

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摘要: Poly-DL-lactide (PLA) and poly-DL-lactide-poly(ethylene glycol) (PELA) were produced by bulk ring-opening polymerization using stannous chloride as initiator. PLA, PELA microspheres, microspheres containing the outer membrane protein (OMP) of Leptospira interrogans with size 1.5–2 μm prepared a solvent evaporation process. In vitro degradation release tests PELA, OMP-loaded performed in pH 7.4 buffer solution at 37°C. Quantitatively, degree was monitored detecting molecular weight reduction, evaluating mass loss apparent rate constant, determining intrinsic viscosity poly(ethylene content retrieved polymer, while profile assessed measuring amount presented medium various intervals. Qualitatively, morphological changes observed scanning electron micrography. The relative rates versus reduction are consistent erosion process rather than surface for microspheres. introduction hydrophilic domains copolymer presence OMP within show critical influences on profile. present triphasic close correlation is between polymer profiles. It suggested that rate, diffusion coefficient, water-swollen structure matrix commonly contribute to from © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 78: 140–148,

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