Modeling the effect of exogenous calcium on keratinocyte and HaCat cell proliferation and differentiation using an agent-based computational paradigm.

摘要: In this study we sought to develop a computational modeling paradigm in order describe the influence of calcium on normal and transformed keratinocyte proliferation differentiation. Keratinocytes and HaCat cells were grown monolayer cultures with low physiologic concentrations, and levels of involucrin expression assessed. Both types grew as monolayers under low-calcium environment, stratified media levels calcium. However, keratinocytes more proliferative rather than calcium, whereas the opposite was true for HaCat cells. Normal keratinocytes differentiated increased. HaCat cells showed little differentiation at any concentration. However, while computer simulation could be modified effect growth keratinocytes, our findings did not support hypothesis that simply ‘‘turning off’’ ability differentiate would account characteristics these This demonstrates application of computational testing biological systems