Exploiting MCF-7 Cells’ Calcium Dependence with Interlaced Therapy

摘要: The purpose of this study is to demonstrate MCF-7 cells’ dependence on calcium for growth and exploit that improve chemotherapy efficacy. Fura-2 fluorescence imaging shows cells maintain a higher basal intracellular concentration than non-tumorigenic MCF-10A cells. Blocking T-type channels with mibefradil reduced concentration. Flow cytometry knocking down channel expression siRNA caused an increase in G1 phase decrease S phase. Proliferation assays treated EGTA thapsigargin reveal the cell extracellular sources, respectively. In vitro, interlaced treatment alternated blocker NNC-55-0396 paclitaxel more effectively number alone. mouse vivo model, xenograft size These findings indicate are dependent proliferation, particularly passing G1/S cycle checkpoint. Further, can be exploited by alternating blockers therapy scheme increases efficacy chemotherapy.