T-type Ca2+ Channel Expression in Human Esophageal Carcinomas: A Functional Role in Proliferation

摘要: In the present study role of T-type Ca(2+) channels in cancer cell proliferation was examined. Seventeen human esophageal lines were screened for using RT-PCR and voltage-clamp recordings. mRNAs all three channel alpha(1)-subunits (alpha(1G), alpha(1H), alpha(1I)) detected 17 lines: either alpha(1H) alone, alpha(1G), or alpha(1)-subunits. Eleven further subjected to recordings: one, i.e. TE8 line, found exhibit a typical current while others exhibited minimal no current. Cell assays performed presence absence blocker mibefradil KYSE150, KYSE180 TE1 cells expressing mRNA but lacking current, exhibiting Only reduced by mibefradil. Silencing alpha(1G)-gene that encodes functional with type-specific shRNA transduction also significantly decreased proliferation. The reduction be associated an up-regulation p21(CIP1). Moreover, p53 silencing nearly abolished p21(CIP1) resulting from blockade. Together, these findings suggest certain carcinomas, inhibition reduces via p53-dependent pathway.