Detecting, visualising, and quantifying mucins.

摘要: The extreme size, extensive glycosylation, and gel-forming nature of mucins make them a challenge to work with, methodologies for the detection must take into consideration these features ensure that one obtains both accurate meaningful results. In understanding appreciating mucins, this affords researcher valuable toolkit which can be used full advantage in detecting, quantifying, visualising mucins. employment combinatorial approach mucin detection, using antibody, chemical, lectin methods, allows important information gleaned regarding extent specific species, distribution within given sample. chapter, is guided through considerations structure how affects advantage. Techniques including ELISA, dot/slot blotting, Western use lectins antibodies on membranes as well immunohistochemistry immunofluorescence tissues cells grown Transwell? inserts are described. Notes along with each section advice best practice describe any associated limitations particular technique from further develop protocol.