Up-regulation of calcium-dependent proteolysis in human myoblasts under acute oxidative stress.
作者： Elise Dargelos , Cédric Brulé , Pascal Stuelsatz , Vincent Mouly , Philippe Veschambre
DOI: 10.1016/J.YEXCR.2009.07.025
关键词:
摘要: The reduced regenerative potential of muscle fibres, most likely due to a decreased number and/or function satellite cells, could play significant role in the progression ageing. Accumulation reactive oxygen species has been clearly correlated sarcopenia and contribute impairment cell function. In this work we have investigated effect oxidative stress generated by hydrogen peroxide cultured human skeletal cells. We specifically focused on activity regulation calpains. These calcium-dependent proteases are known regulate many transduction pathways including apoptosis critical our experimental conditions, which induce an increase calcium concentration, protein oxidation apoptotic death, up-regulation calpain expression were observed ATP synthase, major component respiratory chain, was identified as target. Interestingly able protect cells from these H(2)O(2)-induced effects prevent with natural antioxidant extracted pine bark (Oligopin). data strongly suggest that impair functionality via calpain-dependent such Oligopin activation.
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