Comparative proteomic analysis of myotube caveolae after milli-calpain deregulation.
作者： Sébastien Goudenege , Elise Dargelos , Stéphane Claverol , Marc Bonneu , Patrick Cottin
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摘要: Caveolae are specialised RAFTs (detergent-resistant membrane microdomains enriched in cholesterol and glycosphingolipids). Caveolin, the main caveolae protein, is essential to organisation of proteins lipids, interacts with numerous mediating through a 'Caveolin Scalfolding Domain'. Consequently, play major role signal transduction appear be veritable signalling platforms. In muscle cells, for fusion differentiation, also implicated type muscular dystrophy (LGMD1C). preceding work, we demonstrated presence active milli-calpain (m-calpain) myotube caveolae. Calpains calcium-dependent proteases involved several cellular processes, including myoblast migration, PKC-mediated intracellular remodelling cytoskeleton. For first time, have proved cholesterol-dependent localisation m-calpain C 2 12 myotubes. Calpain-dependent involvement was strongly suggested. Furthermore, eight differentially expressed associated were identified by 2-DE LC-MS/MS analyses using an antisense strategy. This proteomic study demonstrates action on vimentin, desmin vinculin suggests m-calpain's mitochondrial pathways.
sci-hub.se 参考文章(36)
R.G. Anderson, P. Liu, B.K. Pilcher, W.P. Li, Cell-specific targeting of caveolin-1 to caveolae, secretory vesicles, cytoplasm or mitochondria. Journal of Cell Science. ,vol. 114, pp. 1397- 1408 ,(2001) , 10.1242/JCS.114.7.1397
Cristina Capanni, Patrizia Sabatelli, Elisabetta Mattioli, Andrea Ognibene, Marta Columbaro, Giovanna Lattanzi, Luciano Merlini, Carlo Minetti, Nadir M Maraldi, Stefano Squarzoni, Dysferlin in a hyperCKaemic patient with caveolin 3 mutation and in C2C12 cells after p38 MAP kinase inhibition. Experimental and Molecular Medicine. ,vol. 35, pp. 538- 544 ,(2003) , 10.1038/EMM.2003.70
E. Ikonen, S. Heino, S. Lusa, Caveolins and membrane cholesterol Biochemical Society Transactions. ,vol. 32, pp. 121- 123 ,(2004) , 10.1042/BST0320121
Gayle M. Smythe, Thomas A. Rando, Altered caveolin-3 expression disrupts PI(3) kinase signaling leading to death of cultured muscle cells. Experimental Cell Research. ,vol. 312, pp. 2816- 2825 ,(2006) , 10.1016/J.YEXCR.2006.05.010
Sébastien Goudenege, Sylvie Poussard, Sandrine Dulong, Patrick Cottin, Biologically active milli-calpain associated with caveolae is involved in a spatially compartmentalised signalling involving protein kinase C alpha and myristoylated alanine-rich C-kinase substrate (MARCKS) The International Journal of Biochemistry & Cell Biology. ,vol. 37, pp. 1900- 1910 ,(2005) , 10.1016/J.BIOCEL.2005.04.010
Daniela Volonte, Aaron J. Peoples, Ferruccio Galbiati, Modulation of myoblast fusion by caveolin-3 in dystrophic skeletal muscle cells: implications for Duchenne muscular dystrophy and limb-girdle muscular dystrophy-1C. Molecular Biology of the Cell. ,vol. 14, pp. 4075- 4088 ,(2003) , 10.1091/MBC.E03-03-0161
Andrew J Halayko, Gerald L Stelmack, The association of caveolae, actin, and the dystrophin-glycoprotein complex: a role in smooth muscle phenotype and function? Canadian Journal of Physiology and Pharmacology. ,vol. 83, pp. 877- 891 ,(2005) , 10.1139/Y05-107
Christopher M Hosfield, John S Elce, Peter L Davies, Zongchao Jia, Crystal structure of calpain reveals the structural basis for Ca2+-dependent protease activity and a novel mode of enzyme activation The EMBO Journal. ,vol. 18, pp. 6880- 6889 ,(1999) , 10.1093/EMBOJ/18.24.6880
Ki-Bum Kim, Joong-Won Lee, Chang Seok Lee, Bong-Woo Kim, Hyo-Jung Choo, Soon-Young Jung, Sung-Gil Chi, Young-Sil Yoon, Gyesoon Yoon, Young-Gyu Ko, Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts. Proteomics. ,vol. 6, pp. 2444- 2453 ,(2006) , 10.1002/PMIC.200500574
Isabelle Richard, Odile Broux, Valéerie Allamand, Françoise Fougerousse, Nuchanard Chiannilkulchai, Nathalie Bourg, Lydie Brenguier, Catherine Devaud, Patricia Pasturaud, Carinne Roudaut, Dominique Hillaire, Maria-Rita Passos-Bueno, Mayana Zatz, Jay A Tischfield, Michel Fardeau, Charles E Jackson, Daniel Cohen, Jacques S Beckmann, Mutations in the proteolytic enzyme calpain 3 cause limb-girdle muscular dystrophy type 2A Cell. ,vol. 81, pp. 27- 40 ,(1995) , 10.1016/0092-8674(95)90368-2