Survey of Gene Amplifications during Prostate Cancer Progression by High-Throughput Fluorescence in Situ Hybridization on Tissue Microarrays

摘要: Prostate cancer development and progression is driven by the accumulation of genetic changes, nature which remains incompletely understood. To facilitate high-throughput analysis molecular events taking place in primary, recurrent, metastatic prostate cancer, we constructed a tissue microarray containing small 0.6-mm cylindrical samples acquired from 371 formalin-fixed blocks, including benign prostatic hyperplasia ( n = 32) primary tumors 223), as well both locally recurrent 54) metastases 62) patients with hormone-refractory disease. Fluorescence situ hybridization (FISH) was applied to consecutive sections probes for five different genes. High-level (≥3X) amplifications were very rare