A comparison of the Sensititre® MYCOTB panel and the agar proportion method for the susceptibility testing of Mycobacterium tuberculosis.

摘要: The agar proportion method (APM) for determining Mycobacterium tuberculosis susceptibilities is a qualitative that requires 21 days in order to produce the results. Sensititre allows quantitative assessment. Our objective was compare accuracy, time results, and ease of use APM. 7H10 plates APM 96-well microtiter dry MYCOTB panels containing 12 antibiotics at full dilution ranges were inoculated with M. read colony growth. Thirty-seven clinical isolates tested using both methods 26 challenge strains blinded only. displayed 99.3% concordance provided reliable results on day 21, whereas consistent by 10. provides more rapid, quantitative, efficient testing first- second-line drugs when compared gold standard. It will give clinicians sense degree susceptibility, thus, guiding therapeutic decision-making process. Furthermore, microwell plate format without need instrumentation allow its resource-poor settings.