Isolation of a major protein component of Rosenthal fibers.

摘要: Rosenthal fibers (RFs) are inclusions within astrocytes, characteristic of Alexander's disease, but also seen in astrocytic tumors and occasionally glial scar tissue. They granular deposits, intimately associated with intermediate filaments. Their composition has been unknown. The authors have isolated a protein about 19 kd from partially purified RFs disease central nervous system Antibodies were raised to this shown react it on nitrocellulose blots bind tissue sections. Small amounts detected normal brain cultured rat astrocytes. Charge heterogeneity was inferred because several species separated by isoelectric focusing.