Rosenthal fibers share epitopes with alpha B-crystallin, glial fibrillary acidic protein, and ubiquitin, but not with vimentin. Immunoelectron microscopy with colloidal gold.

摘要: Ultrastructural immunoreactivities of alpha B-crystallin, glial fibrillary acidic protein (GFAP), ubiquitin, and vimentin in Rosenthal fibers (RFs) isolated from an Alexander's disease brain were investigated using nonosmium low-temperature embedding technique. The morphology RFs embedded Lowicryl K4M resin was well preserved after treatment with 0.5% Triton X-100. B-crystallin immunoreactivity present various sizes the strongest loosely scattered deposits, which considered to be initial stage RFs. Glial heavy, homogeneous throughout RFs, equivalent that networks filaments. Immunoreactivities both GFAP mainly restricted high electron-dense areas within proved exist close each other by double immunolabeling. negative for vimentin. Ubiquitin relatively small diameters, but large diminished center. Based on these observations, combined tendency self-aggregation it is conceivable are huge aggregation products involving GFAP, ubiquitination may a consequent phenomenon, as intracytoplasmic inclusions, such neurofibrillary tangles Lewy bodies.