Molecular Cloning and Expression of a Phosphoinositide-specific Phospholipase C of Dictyostelium discoideum
作者： A.L. Drayer , P.J. van Haastert
DOI: 10.1016/S0021-9258(19)36974-1
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摘要: A number of phosphoinositide-specific phospholipases C (PLC) different species have recently been cloned. The predicted amino acid sequences these isoforms contain two highly conserved domains. Here we report the identification a PLC gene Dictyostelium by using polymerase chain reaction. Primers were designed coding for regions located within one domains PLCs. Cloning and sequencing reaction product revealed unique PLC-like sequence. This sequence was used to screen library isolate several overlapping cDNA clones. complete expressed in cells resulting increased basal levels inositol 1,4,5-trisphosphate enhanced activity. identified PLC, DdPLC, encodes protein with calculated molecular mass 91 kDa. deduced contains found other isoforms, separated short variable region. C-terminal part shows strong homology mammalian PLC-delta isoform. DdPLC is at all stages development, an increase transcription during starvation culminating fruiting body.
narcis.nl参考文章(33)
D. J. Watts, J. M. Ashworth, Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture Biochemical Journal. ,vol. 119, pp. 171- 174 ,(1970) , 10.1042/BJ1190171
P J M Van Haastert, M J De Vries, L C Penning, E Roovers, J Van der Kaay, C Erneux, M M Van Lookeren Campagne, Chemoattractant and guanosine 5′-[γ-thio]triphosphate induce the accumulation of inositol 1,4,5-trisphosphate in Dictyostelium cells that are labelled with [3H]inositol by electroporation Biochemical Journal. ,vol. 258, pp. 577- 586 ,(1989) , 10.1042/BJ2580577
R.D. Shortridge, J. Yoon, C.R. Lending, B.T. Bloomquist, M.H. Perdew, W.L. Pak, A Drosophila phospholipase C gene that is expressed in the central nervous system. Journal of Biological Chemistry. ,vol. 266, pp. 12474- 12480 ,(1991) , 10.1016/S0021-9258(18)98923-4
T A Tompkins, M A Moscarello, A 57-kDa phosphatidylinositol-specific phospholipase C from bovine brain. Journal of Biological Chemistry. ,vol. 266, pp. 4228- 4236 ,(1991) , 10.1016/S0021-9258(20)64311-3
T. Takenawa, Y. Nagai, Purification of phosphatidylinositol-specific phospholipase C from rat liver. Journal of Biological Chemistry. ,vol. 256, pp. 6769- 6775 ,(1981) , 10.1016/S0021-9258(19)69058-7
Peter J. M. HAASTERT, Pim M. W. JANSSENS, Christophe ERNEUX, Sensory transduction in eukaryotes FEBS Journal. ,vol. 195, pp. 289- 303 ,(1991) , 10.1111/J.1432-1033.1991.TB15706.X
W. Nellen, S. Datta, C. Reymond, A. Sivertsen, S. Mann, T. Crowley, R.A. Firtel, Chapter 4 Molecular Biology in Dictyostelium: Tools and Applications Methods in Cell Biology. ,vol. 28, pp. 67- 100 ,(1987) , 10.1016/S0091-679X(08)61637-4
P M Janssens, P J Van Haastert, Molecular basis of transmembrane signal transduction in Dictyostelium discoideum. Microbiological Research. ,vol. 51, pp. 396- 418 ,(1987) , 10.1128/MR.51.4.396-418.1987
W H Burgess, C A Dionne, J Kaplow, R Mudd, R Friesel, A Zilberstein, J Schlessinger, M Jaye, Characterization and cDNA cloning of phospholipase C-gamma, a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Molecular and Cellular Biology. ,vol. 10, pp. 4770- 4777 ,(1990) , 10.1128/MCB.10.9.4770
Shigeo Ohta, Akira Matsui, Yoshinori Nazawa, Yasuo Kagawa, Complete cDNA encoding a putative phospholipase C from transformed human lymphocytes FEBS Letters. ,vol. 242, pp. 31- 35 ,(1988) , 10.1016/0014-5793(88)80979-7