A Novel Brainstem Hemorrhage Model by Autologous Blood Infusion in Rat: White Matter Injury, Magnetic Resonance Imaging, and Neurobehavioral Features.

摘要: Background Primary brainstem hemorrhage (BSH) has the highest mortality and morbidity as a subtype of intracerebral hemorrhage. A major limitation BSH research is lack corresponding animal model. The purpose this study was to establish novel rat model characterize resulting brain injury, especially focusing on white matter injury. Methods produced by stereotactically injecting autologous whole blood into pons. Time course hematoma resolution observed 7-T magnetic resonance imaging. White injury evaluated in detail multiple parameters including diffuse tensor imaging (DTI), demyelination, axonal oligodendrocyte degeneration, precursor cell proliferation. Brain water content neurobehavior were also evaluated. Results Blood infusion (30 µL) led stable, reproducible right basotegmental absorption started, became obvious, nearly completed at 7, 14, 30 days, respectively. Hematoma caused obvious edema 3 days. mater pathologically, which line with decreased fractional anisotropy (FA) DTI FA reduction noticed cerebral peduncle medulla. Behavioral abnormality persisted for least 14 days neurofunction recovered within 1 month. Conclusions This can produce stable edema, neurofunctional deficits, could be useful future investigation pathophysiological mechanisms new treatment evaluation after BSH.